NH2-Terminal Formylmethionine- and NH2-Terminal Methionine-Cleaving Enzymes in Rabbits

NH2-terminal amino acids of the serum lipoproteins of normal and hypercholesterolemic rabbits.

NH2-Terminal Pro–Brain Natriuretic Peptide and Risk of Diabetes

Brain natriuretic peptide (BNP) has an established role in cardiovascular disease (CVD). However, recent animal studies suggest direct metabolic effects of BNP. To determine the association of BNP with the risk of diabetes, we conducted a prospective analysis of participants from the Atherosclerosis Risk in Communities (ARIC) study. We included 7,822 men and women without history of diabetes, C...

NH2-terminal processing of Dictyostelium discoideum actin in vitro.

When Dictyostelium discoideum actin is synthesized in a rabbit reticulocyte lysate system, it is made as a 43,000-dalton polypeptide with the NHz-terminal sequence Ac-Met-Asp-Gly . . . even though in vivo the NHz-terminal sequence is Ac-Asp-Gly . . . (Rubenstein, P., Smith, P., Deuchler, J., and Redman, K. (1981) J. Biol. Chem 256,8149-8155). Here we describe the subsequent fate of the NHz-term...

In vivo NH2-terminal acetylation of Sindbis virus proteins.

The in vivo incorporation of exogenous radioactive acetate into two proteins of Sindbis virus, the capsid protein and PE2, is described. Under appropriate labeling conditions, 40-50% of the label in the capsid protein is found in an N-acetyl group which constitutes the NH2-terminal modification of this blocked protein. The incorporated radiolabeled acetate was useful in the purification and ana...

Activation of c-Jun NH2-terminal kinase during islet isolation.

Pancreatic islet transplantation has been remarkably improved by the Edmonton protocol; however, it is not easy to achieve insulin independence after islet transplantation from one donor pancreas. The islet isolation procedure itself destroys cellular and noncellular components of the pancreas that probably play a role in supporting islet survival. Further islet transplantation exposes cells to...